Abstract :In this paper, the industrial production of hybrid enzymes was used to deinked the waste paper, which included old messages mixed with coated paper by UV (the ratio of 2:1) and a single UV ink coating of paper. Experiments were carried out with enzyme treatment according to the orthogonal methods. Then, the physical properties (mainly whiteness and residual ink volume) of finished paper were examined. The results showed that the samples treated by enzyme obtained higher brightness and less residual ink as the industrial dosage respectively. The optimum condition for deinking of OMG mixed with UV ink coating of paper (the ratio of 2:1) was: enzyme treatment time is 30 minutes, pH = 9, temperature was 60 ℃, and the dosage of enzyme was 0.3% based on the absolutely weight of fiber. For the single coated paper by UV ink, when the dosage of enzyme was 0.3%, pH value 9, treating time 30 minutes and temperature 50℃, the idealist result was obtained. Treating time and the dosage of enzyme were the main factors which influence the results, and reacting temperature and pH value were secondary factors.