咖啡因、茶多酚以及茄尼醇的分析测定

生物工程论文编号:SW190  论文字数:11241,页数:24

目录
中文摘要 I
英文摘要 II
目录 III
1 绪论 1
1.1选题背景 1
1.2研究进展 3
1.3发展前景 6
2 实验部分 7
2.1 实验材料 7
2.1.1 实验药品 7
2.1.2 试剂制备 7
2.1.2 实验器材 8
2.2  对茶叶以及萃取的烟叶提取物分析方法 8
2.2.1 标准咖啡因曲线的制作 8
2.2.2 样品中咖啡因试液制备及分析 8
2.2.3 样品中茶多酚试液制备及分析 9
2.2.4 茄尼醇标准溶液及样品的分析 9
3 实验结果与讨论 11
3.1 咖啡因标准曲线绘制 11
3.2 咖啡因正交实验结果测定及讨论 11
3.2.1咖啡因分析数据处理 11
3.2.2 咖啡因正交实验结果讨论 12
3.3 茶多酚的实验数据处理及讨论 12
3.3.1茶多酚实验数据处理 12
3.3.2 茶多酚实验结果讨论 13
3.4 茄尼醇标准曲线绘制 13
3.5 茄尼醇正交实验结果测定及讨论 14
3.5.1茄尼醇实验数据处理 14
3.5.2茄尼醇实验结果讨论 15
4 结论与展望 16
致  谢 18
参考文献 19

摘要： 咖啡碱不仅是构成茶汤滋味的重要组分,而且具有强心、利尿、解毒、平喘等药理功能,是一种紧俏的生物碱类医用原料。茄尼醇具有较强的吸收自由基的功能，同时也是合成治疗心血管等药物的中间体。本文对超临界CO2流体萃取后的茶叶以及从烟叶中萃取出来的茄尼醇初品进行分析，测定茶叶中咖啡因的脱除率为85%时的工艺条件为含水量为40%，萃取时间为8小时，萃取温度为50℃,在这个工艺条件下萃取后的茶叶中茶多酚脱出率极小而经萃取出来的茄尼醇粗品中的含量也初步达到了21.60%，其萃取条件为萃取时间2h、萃取温度50℃、萃取压力20 MPa、夹带剂乙醇体积分数为95%,为工业化生产提供经验。

关键词：咖啡因；茶多酚；茄尼醇；分析

Abstract: Caffeine not only is an important composition which makes up of the taste of tea soup, also has pharmacology functions as tonify the heart, diuresis, detoxification, preventing asthma. it is an alkaloid medical material in great demand. Solanesol has the functions as absorb free radical, it also is the intermediate in the synthesis of drugs which can cure cardiovascular disease.In the article, we analyze the composition of tea and tobacco leaf after Supercritica fluid extraction(SFE) extraction, when the rate of determination of caffeine is 85% in tea,which process conditions for the water content of 40%, extraction time to eight hours, the temperature was 50°C. In this condition after the extraction of polyphenols in tea minimal extrusion rate , By the extraction of solanesol crude  the content of the initial has reached 21.60%. While after the conditions of its extraction time 2 h, extraction temperature 50°C, the extraction pressure 20 MPa, cosolvent ethanol volume fraction of 95%. Which offer the industrial production experience.

Key words: Caffeine; Catechins; Solanesol; Analysis