一株微生物新种生物学特性研究

生物工程论文编号:SW159  论文字数:13766,页数:30

目录
摘要 I
Abstract II
目录 III
1  绪论 1
1.1  微生物的发展 2
1.2  色素在食品中的应用 3
1.3  生物矿化的特点 4
2  材料与方法 6
2.1  实验材料 6
2.2 方法 7
3  实验结果 12
3.1  菌种的分离纯化 12
3.2  不同固体培养基中菌体生长情况的比较 13
3.3  不同液体培养基中菌体生长情况的比较 14
3.4  培养温度对菌体生长的影响 14
3.5  pH对菌体生长的影响 15
3.6  He1产酶定性分析 16
3.7  抑菌作用 17
3.8  沙土管及其周围沙土失重情况分析 17
3.9  沙土管及其周围沙土中金属元素的分析 18
3.10  He1生物矿化作用分析 21
4  总结与展望 24
致谢 25
参考文献 26

摘要：宁夏沙漠地带发现一种由微生物生长而凝结成的沙土管，对该微生物进行了分离纯化，得到了该微生物的纯培养，并命名为He1。对菌种的生长特性：对该菌的生长培养基、生长温度、菌丝形状、菌落形态等进行研究发现，该菌种的所需的生长培养基是查氏培养基，生长温度为30℃，在显微镜下观察到，菌种He1具有较长的菌丝，菌丝相互交缠，有横隔，成竹节状，基本无分支，有大量孢子产生，查资料确定该菌为放线菌。该菌能产大量的黑色素，在显微镜下，菌丝呈褐色。对提纯的菌种进行生长特性、产酶特点、抑菌作用分析发现该菌蛋白质水解能力很差却具有良好的淀粉水解能力。沙土管金属元素的分析中可以看出，沙土管中的Ca离子含量相当多，沙土管中K 、Ca、Na、Mg金属的含量分别是78.3 mg/kg、90.28 mg/kg、32.19 mg/kg、35.83 mg/kg，而在沙土中K 、Ca、Na、Mg金属的含量分别是57.12mg/kg、20.66 mg/kg、18.59 mg/kg、11.78 mg/kg, Ca离子含量相差4倍多；生物矿化的模拟中在显微镜观察到晶体颗粒，是CaCO3 晶体。

关键词：沙土管；菌种分离纯化；菌种的生长特性；生物矿化

Abstract：Our district that is in the desert in Ningxia discovers that a species may be a new microorganism, so carry on separating the early of the purification plate and picking a single colony to the germ species to grow with the purification germ.To the growth characteristic that germ grow:Carry on the research detection to the growth medium, the growth temperature, hypha shape, the colony appearance etc. of that germ, the growth medium need of that germ species is to check the surname medium, the growth temperature is 30℃, observing under the microscope, the germ species He1 has the longer hypha, the hypha hands over to tie up mutually, is horizontal separate, become the bamboo node form, basic have no branch, there is a great deal of spore creation, checking that germ of the data assurance as actinomycetes.That germ can produce a great deal of melanin, under the microscope, the hypha presents the brown.Have the good starch  water solution ability towards lifting the pure germ species to carry on growing the characteristic and producing the enzyme characteristics, the bacteriostasis analysis to discover that germ protein water  solution ability badly but.The analysis of the sandy soil tube metals chemical element in can see, the Ca ion content that is in the sandy soil tube is very many, the content of the K, Ca, Na, the Mg metals is the 78.3 mgs/ kg,90.28 mgs/ kg,32.19 mgs/ kg,35.83 mgs/ kg respectively in the sandy soil tube, but in the sandy soil the content of the K, Ca, Na, the Mg metals is the 57.12 mgs/ kg,20.66 mgs/ kg,18.59 mgs/ kg,11.78 mgs/ kg respectively, the Ca ion content differ 4 many;The organism mineral turns in the emulation to observe the crystal grain in the microscope, is a crystal of CaCO3.

Keywords: The sandy soil tube；The erm pecies solation purification;The growth characteristic of the erm species; The organism mineral turn