内生真菌HCCB3616的分类鉴定

生物工程论文编号:SW141  论文字数:9304,页数:25

目  录
中文摘要 I
英文摘要 II
目录 III
1. 绪论 1
 1.1  植物内生菌概况 1
 1.2 PCR原理 4 
 1.3琼脂糖凝胶电泳原理 5
 1.4立题依据 5
2. 实验部分 7
 2.1试验材料 7
 2.2试验方法 8
3.结果与讨论 12
 3.1菌株HCCB3616的生长速度考察 12
 3.2菌株的形态、培养特征 13
 3.3 HCCB3616染色体DNA的制备及18S rDNA序列的PCR 15
 3.4 菌株HCCB3616的系统发育分析及鉴定结果 14
4．总结与展望 17
 4.1总结 17
 4.2.展望 17
致谢 19
参考文献 20

摘 要： 本论文利用上海来益生物药物研发中心的植物内生菌资源，通过抗菌模型的筛选，获得多株活性功能菌株，对其中一株活性较高的HCCB3616进行了菌种鉴定。主要研究工作包括以下几个方面：
 对内生真菌HCCB3616进行分离和培养，观察菌体的形态特征。提取它的染色体DNA，进行PCR扩增，以确定其种属分类地位。
 本文采用18S rDNA序列测定与传统形态学观察相结合的方法，对活性菌株HCCB3616进行分类鉴定，各种指标鉴定其为链格孢霉属（Alternaria sp.）。
 
关键词: 内生真菌；抗菌；筛选；18S rDNA；鉴定

Assort and identification of the Endophytic Fungus HCCB3616

Abstract:  In this research work, from a large amount of endophytic fungi which were conserved in Shanghai Health Creation Center for Biopharmaceutical R&D had been screened based on the anti-bacterial model, dozens of strains showed activities. The strain HCCB3616 was taxonomically identified. The research work was as follows:
 Isolate and culture the endophytic fungi HCCB3616,then observe its morphological characteristics. Extract its Chromosomal DNA for PCR amplification to determine its taxonomic status of species.
 By using the methods of 18S rDNA sequences determination combined with morphological observation, the endophytic fungus HCCB3616 was identified as Alternaria sp.
 
Keywords：Endophytic Fungi; Anti-bacterial; 18S rDNA; Identification