枯草芽孢杆菌微胶囊的制备及其稳定性研究

生物工程论文编号:SW127  论文字数:16116,页数:37

目  录
中文摘要 Ⅰ
英文摘要 Ⅱ
目录 Ⅲ
1. 绪论 1
 1.1 微胶囊技术的作用 1
 1.2 微胶囊的制备方法 3
2 实验部分 9
 2.1 菌种 10
 2.2 培养基 10
 2.3 微胶囊芯材制备 10
 2.4 微胶囊的制备方法 11
 2.5 微胶囊品质检验方法 13
3 实验结果与讨论 14
 3.1 芯材的选择 14
 3.2 固定化的选择 15
 3.3 包埋率的测定 15
 3.4 微胶囊粒径 19
 3.5 微胶囊稳定性的分析 23
 3.6 乳化剂的影响 25
 3.7 微胶囊图片展示 25
4 总结与展望 29
致　　谢 31
参考文献 32

 摘 要：本研究利用明胶、壳聚糖、海藻酸钠、黄原胶等作为微胶囊囊材并分别利用锐孔法、倾注法制备单凝聚以及复凝聚微胶囊。分析了利用枯草芽孢杆菌菌体以及芽孢作为微胶囊芯材的优劣,并且优化了促芽孢培养基。实验分别从微胶囊制备方法、包埋率、机械性能、微胶囊稳定性以及菌体稳定性等几个方面考虑,最后得到分别以2.0%壳聚糖；2.5%壳聚糖；2.0%壳聚糖+0.5%黄原胶；这三组作为囊材制备得到的微胶囊最佳。实验也得到培养基:牛肉膏12g；氯化钠5g；水 1000ml；MnSO4 0.5g；pH7.0-7.2,能够最大限度的促进枯草芽孢杆菌产芽孢。
 关键词：枯草芽孢杆菌；微胶囊；制备；芽孢；研究

Bacillus subtilis Microcapsule Preparation and Stability Analysis
Abstract：In this study, gelatin、chitosan、sodium alginate、xanthan gum as the capsule material microcapsules. Analysis of the use of Bacillus subtilis and Bacillus microcapsule core material as the advantages and disadvantages, and to optimize the culture medium to promote Bacillus. xperiments were prepared from the Microcapsule Preparation、 encapsulation rate、 mechanical properties、 micro-capsules, such as stability and the stability of several aspects of cell considered.End 2.0% chitosan;2.5% chitosan;2.0% chitosan and 0.5% Xanthan Gum; These three is the best material of microcapsules. Experiments have also been promoting the growth of Bacillus medium.
 Keywords：Bacillus subtilis; Microcapsule; Preparation; Bacillus; Research