米多霉素胞嘧啶衍生物的分离纯化研究

生物工程论文编号:SW111  论文字数:15956,页数:34

目录
1 绪论 4
 1.1 前言 4
 1.2 抗生素分离纯化的研究概况 5
 1.3 米多霉素类化合物的性质与作用 9
2 实验部分 11
 2.1 实验材料 11
 2.2 实验内容与方法 14
3 结果与讨论 20
 3.1 离子交换树脂的静态筛选 20
 3.2 pH值对DK110树脂吸附容量的影响 22
 3.3 离子交换等温线 23
 3.4 离子交换树脂穿透曲线的测定 25
 3.5 洗脱工艺 26
 3.6 脱色工艺 29
4 总结与展望 32
致 谢 33
参考文献 34

摘要：新型核苷类农用抗生素米多霉素胞嘧啶衍生物（Mildiomycin cytosine analogue，MIL-C）对各种植物的粉状霉菌有强烈的拮抗作用，并且毒性较低。本文主要探索了用离子交换法从发酵液中分离MIL-C的工艺。通过静态筛选，筛选到对MIL-C具有较大静态交换容量和良好吸附过程动力学性能的大孔弱酸性阳离子交换树脂DK110。实验过程考察了不同pH、温度、上样浓度以及上样速度对DK110吸附过程的影响。实验结果表明温度和上样浓度对DK110吸附过程影响不大，实验操作温度可为室温。上样发酵液的pH和流速分别控制在7.0和2BV/h。同时也对洗脱条件进行了优化，确定最佳操作条件为：洗脱流速2BV/h，洗脱剂为2%氨水。另外对MIL-C发酵液的脱色工艺进行了考察，结果表明可选用阴离子树脂D261在pH7.0进行脱色，接触时间约为120min。该脱色工艺的回收率达到了84.71%。
关键词：米多霉素胞嘧啶衍生物；离子交换法；DK110树脂

Abstract: Mildiomycin cytosine analogue (MIL-C), a new nucleoside antibiotic, showed remarkable biological activity against powdery mildews of many plants with low toxicity. This dissertation will pay attention to the process of separating MIL-C from fermentation broth. MIL-C is expected to be separated by ion exchange resins. A weakly cationic exchange resin DK110 was selected from 8 different resins, which showed high static exchange capability and good dynamic character. The effect of pH, temperature, inlet concentration, flow rate to the process of adsorption was studied in this experiment. The temperature for adsorption process was selected as 25℃ because the exchange capability is not affected by the temperament and the inlet concentration. The optimized adsorption was obtained at pH 7.0, flow rate 2BV/h. The elution conditions were determined as follows: 2% NH3·H20 solution, elution flow rate 2BV/h. An effective technology to decolorize the fermentation broth was investigated in this thesis. D261 resin, a kind of anionic exchange resin, was selected and the discoloring conditions were optimized as follows: pH7.0, discoloring time 120 min. Under these conditions, the recovery efficiency achieved 84.71%.
 Key words: Mildiomycin cytosine analogue; Ion exchange; DK110 resin