茶渣水解及产物提取的初步研究

生物工程论文编号:SW109  论文字数:10871,页数:32

目  录
中文摘要 I
英文摘要 II
目  录 III
1  绪论 1
1.1  前言 1
 1.2  茶渣可利用成分 1
 1.3  茶渣的综合利用 2
 1.4  实验目的 3
2.实验部分 4
2.1  实验材料与试剂 4
2.2  实验设备 4
2.3  实验方法与设计 5
2.4  葡萄糖和木糖标准曲线的制作 7
2.5  稀酸水解茶渣的单因素研究 8
3 结果与讨论 9
3.1  料液比对水解结果的影响 9
3.2  稀硫酸浓度对水解结果的影响 9
3.3  稀硫酸水解温度对水解结果的影响 10
3.4  水解时间对水解结果的影响 11
3.5  响应面实验优化水解条件 12
3.6 茶渣脱蛋白结果与分析 18
3.7 茶渣灰分含量测定结果 19
3.8 小结 19
4 总结与展望 19
致  谢 21
参考文献 22
附录 24

 摘 要：随着茶产业的发展，茶渣大量出现在我们的生活中，面对如何处理茶渣的问题，本文对稀酸水解茶渣的工艺条件进行优化。在单因素试验基础上选取试验因素与水平,根据Box-Benhnken的中心组合试验设计原理对试验进行设计和结果分析。研究了温度、时间、稀酸度和料液比对水解的联合影响,得出最佳工艺条件:还原糖水解最佳条件为水解温度120℃、水解时间60min、稀酸度3.0%、料液比8.1%。戊糖水解最佳条件为水解温度120℃、水解时间60min、稀酸度3.0%、料液比13%。

关键词：茶渣；水解；响应面；提取

A Priliminary Study on hydrolysis and extract the products of Tea Residue

Abstract: With the development of tea industry, there were a large number of tea residue appear in our daily life. The optimize conditions of dilute acid hydrolysis was researched to deal with the problem. On the basis of single factor experiments, the combined effects of temperature, dilute-acid concentration, water - solid ratio were investigated and optimized using RSM. The optimum conditions were as follows: reducing sugar’s are reaction temperature 120℃, reaction time 60min, dilute-acid concentration 3%,water - solid ratio 8.1g/L;pentose’s are reaction temperature 120℃, reaction time 60min, dilute-acid concentration 3%,water - solid ratio 13g/100ml.

Keywords： tea-leaf; hydrolyze; response surface methodology; extract