羊肚菌深层发酵和多糖提取分析

生物工程论文编号:SW098  论文字数:10174,页数:30

目录
中文摘要 Ⅰ
英文摘要 Ⅱ
1.绪论 1
 1.1羊肚菌概述 1
 1.2多糖的分离方法简介 2
 1.3 多糖的测定 4
 1.4 羊肚菌发酵的影响因素 5
 1.5研究现状分析与项目意义 6
2．实验部分 7
 2.1  实验材料 7
 2.2  实验方法 8
3.结果与讨论 13
 3.1葡萄糖标准曲线 13
 3.2发酵条件的确定 13
 3.3氨基酸的检测 15
 3.4单糖的检测 16
4.总结与展望 21
 4.1实验总结 21
 4.2展望 22
致  谢 23
参考文献 24

摘要：羊肚菌是一种十分重要的食药兼用真菌，含有丰富的生理活性物质，多糖是其中的重要一种，具有增强肌体免疫力、抗疲劳、抗病毒、抑制肿瘤等多种功能。本实验羊肚菌液体发酵最适发酵温度为25℃，最适的转速为150rpm；溶解氧会抑制菌丝体的生物量和多糖和积累；分离菌丝体和发酵液，然后采用超声破壁，1:40的浸提比浸提3h，浸提次数为两次，用95%的乙醇醇析2d，再用无水乙醇洗涤两次、Sevage法脱蛋白后用ΦMW3500 透析制取胞内和胞外多糖；采用苯酚-硫酸法测定胞内多糖的总糖，最高的得率有3.5%；液相分析结果表明，胞内还有游离的单糖，胞外则不含游离的单糖；用2mol/L的硫酸水解后的多糖进行液相分析，结果表明，羊肚菌多糖可能有葡萄糖、半乳糖、甘露糖、木糖。
关键字：羊肚菌；发酵；多糖；单糖；液相色谱法

Deep fermentation fo Morchella and extraction of polysaccharide

Abstract: Morchella is a very important edible and medicinal fungi, is rich in physiologically active substances, polysaccharide is one of the important one, with enhanced body immunity, anti-fatigue, anti-virus, and many other features of tumor. In this experiment, the optimum fermentation fermented morel liquid temperature was 25 ℃, the optimal speed is 150rpm; Dissolved oxygen will inhibit the mycelium biomass and polysaccharide and accumulation; Separation of mycelium and fermentation broth, and then broken using ultrasonic, 1:40 extraction of ratio, extraction 3h, extraction times for the two, with 95% ethanol precipitation 2d, then using ethanol washing twice and suing  Sevage  removal protein , dialysis with ΦMW3500 ,the and Receive the polysaccharide. Liquid chromatography analysis showed that there are free intracellular monosaccharide, is non-free extracellular monosaccharide; With 2mol / L sulfuric acid hydrolysis of the polysaccharide Liquid chromatography analysis, the results show that the polysaccharides may be glucose, galactose, mannose, xylose.
Keywords: Morchella; Fermentation; Polysaccharide; Single sugar; Liquid chromatography