关键词:异硫氰酸盐; 云苔属蔬菜; 种子; 高效液相色谱(HPLC); 气相/质谱联用(GC-MS)
Extraction and analysis of isothiocyanates from brassica species
Abstract: Isothiocyanates, which are rich in Cruciferae family, have been shown to have anticarcinogenesis. In this paper, the extraction technique parameters of isothiocyanates were studied. The results showed that the extracting rate of techniqueⅠwas the highest, comparing with other technique, so the extraction technique Ⅰ was used in the afterwards experimentation. Glucosinolates autolysis products of seed meal were extracted from hydrolyzate of brassica species by methylene chloride method and then analyzed by gas chromatography-mass spectrometry. More than 12 compounds were identified. The main compounds were allyl isothiocyanates, 3-BITC, PEITC, sulforaphane, goitrin, etc,and their content was mensurated. Sulforaphane was found mainly in broccoli, PEITC was found mainly in root mustard and cauliflower. Allyl isothiocyanate and 3-BITC were found in most species of Brassica L.