关键词:桑葚,总黄酮,正交设计,大孔吸附树脂,分离纯化
Extraction Condition and Research of Total
Flavonoids in Mulberry
Abstract: To study technique condition for separating total flavonoids from mulberry. With orthogonal experiment based on single factor, The total extraction rate of flavonoids for purpose, the effects of ethanol concentration, ratio of material to liquid, extraction temperature, extraction temperature, extraction time and times on extraction of total flavonoids from mulberry were study to obtain the optimal extraction conditions; adsorbance of mulberry total flavonoids for indicators, Choose a suitable macroporous resin, Study Adsorption and Desorption optimum conditions, analysis pH, salt concentration, temperature, ethanol concentration. Mulberry of total flavonoids extracted from optimal conditions, 75% of ethanol, water bath temperature of 75 ℃, extracting liquid ratio of 1:15, extraction time is 2h, pH is 4.0 , salt oconcentration is 3% , temperature is 50℃, ethanol concentration is 50%, elution rate of ethanol is 0.5-1.0ml/min,3 column volume. Mulberry of total flavonoids in the extraction rate can be achieved 2600mg/kg. Under the experimental conditions, total flavonoids extracted from mulberry by D-101 macroporous resin nearly 50%. Choose the D-101macroporous resin, choose the medicine mulberry or black mulberry, and D-101 macroporous resin applied to mulberry of total flavonoids in the separation and purification.
Keywords: Mulberry, total flavonoids, orthogonal design, macroporous resin, separation and purification.