Detection and Extraction Conditions of Luteolin in Perilla
Abstract: Perilla contained many kinds of bio-active substances, one of these substances was Luteolin, which had very strong antioxidant ability.Therefore,
it can be used for antitussive,expectorant,asthma,antispasmodic,antibacterial,anti-tumor, anti-inflammatory,dilate coronary,which is widely used.This test is studying how to extract luteolin from perilla,and the contend of luteolin in different parts of perilla (leaves, stems,seeds)and different local of perilla,using orthogonal test method and HPLC to study different concentrations of solvent and extraction method for the yield,as well as different types of basil in the luteolin content. The results are:the optimal process condition of ethanol extraction method to determineare: 60% ethanol concentration,temperature 55℃,the smallest particles. At the optimum conditions,the maximum luteolin yield is 0.234%. Perilla seed in the highest content of luteolin,the average rate is 0. 279mg/g.
Keywords:Luteolin,Perilla,Orthogonal test method,High Performance Liquid
Chromatography