Abstract:This paper established the reverse phase high performance liquid chromatogra-
phic methods of nitroimidazoles residues detection in animals edible tissues, marine lives, bee products and egg albumen by the means of extemal standard.
The HPLC methods of Nitroimidazoles in animals edible tissues: After an initial extraction with ethyl acetate and evaporation in sequence, the sample was resolved in hydrochloric acid. Hexane was used in the liquid-liquid extraction to remove fat. A MCX solid phase separation was performed and used ammonia and methanol as eluates. Then HPLC analysis carried out as follows: Detecting wavelengh was at 320nm and mobile phase was acetate buffer solution and acetonitrile(86:14,v/v). The limits of detection and quantification for nitroimidazoles were 1.0μg/kg. Recovery ratios of MTZ, DMZ, ONZ, TNZ, RNZ in chicken were 90.8%, 78.8%, 75.1%, 75.2%, 83.2%; in pork tissue were 83.5%,76.1%,71.5%,75.2%,79.3%; in aquatic products were 72.5%, 75.0%,71.5%,72.7%,73.3%; in bee product were 82.6%,73.7%, 76.2%, 73.1%, 80.6%.
The HPLC methods of Nitroimidazoles in eggs albumen: eggs albumen(10g) was put in a centrifuge tube. Isopropanol and chloroform were added, the tissue was dispersed by vortex-mixing and placed in an ultrasonic bath. Finally, after centrifugation the hexane fraction was discarded. The recovery ratios were 87.7%,71.2%,76.6%,73.7%,75.9%.
The results showed that this method was enough sensitive, accurate and precise to deteminate the nitroimidazoles residues and can be applied to determinate nitroimidazoles residues in animal edible tissue.
Key words:Nitroimidazoles; HPLC; animals edible product