Abstract：TNF-α（Tumor necrosis f factor-alpha）is a proinflammatory cytokine, the immune response, inflammation and injury response play an important role, mainly affect cell proliferation and apoptosis of cells, not only to tumor cytotoxicity, cell lysis, inhibition of proliferation and induction of apoptosis, etc, but also a variety of normal cell growth and differentiation with other cytokines together to form a complex immune network.
Using molecular biology PCR, PCR products were purified and inserted connecting carrier (gel) technology, Preparation of competent cell technology, foreign DNA into technology, plasmid extraction, DNA sequencing technology, prokaryotic expression technology, we development anti-TNF-Europium antibody Fab small molecule drugs for the treatment of TNF-Europium role related diseases.
Keywords : Amplified by PCR technology; Gene cloning; E.coli; Fd gene; Recombinant plasmid
