中药强化类胡萝卜素发酵及其产物稳定性研究

生物工程论文编号:SW210  论文字数:12631,页数:32

目 录
中文摘要 I
英文摘要 II
目录 III
1. 绪论 1
 1.1 内容概述 1
 1.2 β-胡萝卜素和番茄红素的结构及理化性质 2
 1.2.1 β-胡萝卜素的结构及理化性质 2
 1.2.2 番茄红素的结构及理化性质 2
 1.3 β-胡萝卜素及番茄红素的生物合成 3
 1.4 β-胡萝卜素及番茄红素的生理功能 ..............................4
 1.5 β-胡萝卜素及番茄红素的研究意义及现状  .......................5
2. 实验部分 5
 2.1 实验材料...................................................... 5
 2.1.1 菌种.....................................................5
 2.1.2 药品或试剂...............................................5
 2.1.3 主要仪器.................................................6
 2.1.4培养基................................................... 6
 2.2 实验方法 .............................................................7
 2.2.1 试验流程.................................................7
 2.2.2 培养条件.................................................7
 2.2.3 测定方法.................................................7
  2.2.4 中药提取方法.............................................8
 2.2.5 实验设计.................................................8
3 .实验结果和讨论 ...................................................10
 3.1不同中药水提液对三孢布拉氏霉菌（-）生长的影响 ................10
 3.2 标准曲线绘制.................................................16
 3.2.1 β-胡萝卜素标准曲线绘制................................16
 3.2.2 番茄红素标准曲线绘制...................................17
 3.3初步筛选出的中药摇瓶发酵结果..................................18
 3.4各因素对粗提物稳定性的影响....................................18
4．总结与展望 .......................................................26
致谢.................................................................27
参考文献.............................................................28

 摘要：本论文通过平板培养结合摇瓶发酵，对21种常用中药进行了大规模筛选，结果表明：在平板上，中药黄芪，灵芝，枸杞子，太子参对三孢布拉氏霉菌生长有一定的促进作用，中药三七，川穹，何首乌，桑寄生，丹参，绞股蓝，杜仲，陈皮，菟丝子，西洋参，积实，制萸肉对三孢布拉氏霉菌生长有一定的抑制作用，其中三七明显促进产色素。其它中药银杏叶，五味子，大枣，麦冬，野菊花则无明显的促进及抑制作用。在平板筛选的基础上，对在平板上有明显促进作用的中药进行了摇瓶复筛，结果表明，黄芪对三孢布拉氏霉菌产β-胡萝卜素、番茄红素等类胡萝卜素有一定的提高。接着通过研究温度，光照，还原剂，氧化剂，酸等因素对粗提物稳定性的影响，表明发酵得到的粗提物应避光冷藏。
 关键词：β-胡萝卜素；番茄红素；三孢布拉氏霉菌；中药

Abstract: Through the combination of plate culture and shake flask fermentation culture, to conduct a large-scale screening with 21 kinds of commonly used Chinese medicine. The results show that  in the plate,Chinese medicine as Astragalus, Ganoderma lucidum, medlar, Pseudostellaria can promote Blakeslea trispora grow well, Chinese medicine as Notoginseng, Chuanqiong, Polygonum multiflorum, Loranthaceae, Danshen, Pentaphyllum，Eucommia, Chenpi, Dodder, American ginseng,Jishi , Zhiyurou have acertain degree of inhibition of growth for Blakeslea trispora, Notoginseng which significantly promote the production of the pigment. Other Chinese medicine ginkgo biloba, schisandrin，Chinese date, Ophiopogon japonicus, wild chrysanthemum have no obvious promotion and inhibition. On the basis of plate culture,have hake flask fermentation culture with the Chinese medicine that have obvious promotion. The results show that Astragalus could enhanced production of β-carotene and lycopene by Blakeslea trispora. Then by studying the temperature, light, reducing agent, oxidizing agents, acids and other factors on the stability of crude extracts from the impact, indicating that fermentation extracts obtained should be frozen and dark.
Keywords：b-carotene; lycopene; Blakeslea trispora; Chinese medicine