Abstract: Through the combination of plate culture and shake flask fermentation culture, to conduct a large-scale screening with 21 kinds of commonly used Chinese medicine. The results show that in the plate,Chinese medicine as Astragalus, Ganoderma lucidum, medlar, Pseudostellaria can promote Blakeslea trispora grow well, Chinese medicine as Notoginseng, Chuanqiong, Polygonum multiflorum, Loranthaceae, Danshen, Pentaphyllum,Eucommia, Chenpi, Dodder, American ginseng,Jishi , Zhiyurou have acertain degree of inhibition of growth for Blakeslea trispora, Notoginseng which significantly promote the production of the pigment. Other Chinese medicine ginkgo biloba, schisandrin,Chinese date, Ophiopogon japonicus, wild chrysanthemum have no obvious promotion and inhibition. On the basis of plate culture,have hake flask fermentation culture with the Chinese medicine that have obvious promotion. The results show that Astragalus could enhanced production of β-carotene and lycopene by Blakeslea trispora. Then by studying the temperature, light, reducing agent, oxidizing agents, acids and other factors on the stability of crude extracts from the impact, indicating that fermentation extracts obtained should be frozen and dark.
Keywords:b-carotene; lycopene; Blakeslea trispora; Chinese medicine