Study of Mutagenic Dosage of 60Co-γ Ray Irradiated on Pleurotus eryngii
Abstract:Pleurotus eryngii is under the fungi door, Pleurotus. its meat hypertrophy like abalone and with almond flavor, which was named after Pleurotus eryngii. Its proteins and polysaccharides content is very rich, and contains a variety of biological substances, is favored by consumers at home and abroad. It is one of the rare varieties of edible fungi. Its production cycle is short and cost-effectiveness is relatively high, in recent years has expanded rapidly.
In order to obtain high-quality, high-yield strains of Pleurotus eryngii, This study using 60Co-γ-rays to radiation on the spores, mycelia, Fruitbody of Pleurotus eryngii, observation of their mutagenic effect. Use Microscope to observation the mycelium and contrast with Control group; Observation the colony morphology of growth contrast with Control group, and do antagonistic experiment to selected mutants.
The experimental results show that best dose of the hyphae of Pleurotus eryngii is 500 Gy; In radiation dose of 200 Gy, the spores died rate is 70%; Fruitbody semi-lethal dose is 1000 Gy. Through Antagonism Testing and Liquid Fermented Mycelia Accumulation Test , found that radiation after the strains compared with the original strain, the accumulation of mycelium difference is not obvious, but preliminary results determine the semi-lethal dose for further study on the lure Pleurotus eryngii Variable breeding provided a theoretical basis.