Sequence Analysis of Cytochrome Oxidase Subunit I
Gene Fragment of Meretrix meretrix
Abstract: The whole DNA of M. meretrix was extracted using CTAB method from adductor muscle. And the COI gene fragment were amplified by PCR and sequenced in Shanghai Sangon. The result showed that size of COI gene fragment was 709bp (contain the primer), the A, T, G and C contents in this fragment were 21.44%, 44.85%, 20.17% and 13.54% respectively. The A+T content was higher than G+C content. The same sequences of 12 Veneridae clams were downloaded from GenBank which have above 74% homology with M. meretrix.The homologus sequences were aligned, and the molecular phylogenetic tree was constructed with the sequence information of petricola lapicida as outgroup using MegAlign software. Tree topologies indicated that M. meretrix shared a close relationship with Meretrix lusoria and Meretrix lamarckii. It is consistent with the morphological and taxonomic results.
Keywords: M. meretrix; Cytochrome Oxidase Subunit I Gene; Sequence analysis.