Investigation of Fish uricase activity
[Abstract] The uricase is a kind of enzyme in the purine degeneration pathways of organism, the uric acid can be oxidated in allantoin, CO2 and H2O2, human does not have the uricase, therefore the uric acid accumulates in the vivo clinical organism such as the pathological change like gout.In order to obtains the medical gout vaccine through using the genetic engineering method, there is need to obtain the most superior expression choice to the uricase gene in which kind of animal model appropriate to express the object.The uric acid enzyme activity can be determinated by the response of enzyme . The expression of the candidate animal model compared with each kind, for finally chooses expresses the object to provide the reference.
[Keyword] Uricase gout vaccine expression targets enzymatic reaction