夫西地酸的分离纯化研究

制药工程论文编号:ZY068    论文字数:7421,页数:20

 目  录
中文摘要 I
英文摘要 II
目录 III
1．绪论 1
1.1夫西地酸概述 2
1.2大孔吸附树脂概述 2
1.3总结 3
2．实验部分 3
2.1夫西地酸标准品在不同溶剂下的溶解度实验 3
2.2夫西地酸发酵液的分离研究 4
2.3夫西地酸的HZ-816树脂柱层析提纯 5
3．结果与讨论 7
3.1夫西地酸标准品在不同溶剂下的溶解度实验 7
3.2夫西地酸发酵液的分离研究结果 7
3.3夫西地酸的HZ-816树脂柱层析提纯结果 9
3.4实验结果总结 12
4．总结与展望 13
4.1总结 13
4.2展望 13
致谢 15
参考文献 16

摘要 :本论文研究建立夫西地酸发酵液的分离纯化工艺，夫西地酸是一种窄谱、高效的抗生素，对预防和治疗葡萄球菌感染疗效显著。根据夫西地酸的溶解特性，采用过滤、干燥、萃取和柱层析等技术相结合，设计不同的工艺路线，通过对酸碱调节试剂、流动相梯度、柱层析树脂的选择，采用高效液相色谱法检测产物的纯度和收率作为评价指标，得到的最优工艺流程。该工艺其产品的收率为44.53%，组分含量为98.23%。该方法简便易行，准确可靠，能有效控制产品的质量，可用于夫西地酸的工业生产。
关键词：夫西地酸；分离；柱层析；高效液相色谱法

Abstract:This study was to establish the technology process of the separation and purification of the fucidic acid. Fusidic acid is an efficient narrow-spectrum antibiotic. The treatment of staphylococcus aureus infection is remarkable. According to the dissolution characteristics of fusidic acid, we used the filtering, drying, extraction, column chromatography and so on to design different technics processes. HPLC was used to check the purity and yield of the products to optimize the production process of the fucidic acid. Results suggested that the yield was 44.53% and the component concentration was 98.23%. This technology process was simple, accurate, reliable and effective for the manufacturing production.
Keywords: Fucidic acid; Separation; Column chromatography; HPLC