关键词:蟾皮;华蟾酥毒基;高效液相色谱法
Abstract:. This article discusses the process of the methanol extraction of toad skin in detail, the use of reagents and equipments, the methodology and operation of the main points., It is said in literature that Dry skin contains Xenopus Bufalin, cinobufotalin ,China Chansu drug-based, which are lactone compounds with small polarity .. Comparing the heating circumfluence extraction with the ultrasonic extraction of methanol , the latter was chioced in the experiment. Through different tests and the analysis of the experimental results, an optimized experiment program was determined. Cinobufotalin as the study goals from extract by HPLC .The mobile phase was determind by contrast the ChP method ( solution of 0.5% potassium dihydrogen phosphate - acetonitrile mobile phase system) with the literature method ( acetonitrile - water mobile phase systems). A better result can be acquired by pharmacopoeia method with adjusting the ratio of mobile phase. The resolution is over 1.5, and theoretical plate number in accordance with Cinobufotalin is not less than 2000.
Keywords:Toad skin ; Cinobufotalin; HPLC