Abstract: By discussing the importance of Fe(II)EDTA in the action of Fe(II)EDTA -NO microbial reduction, the reduction properties of microorganisms under the conditions of shaking flasks and continuous operation, the isolation of microorganisms and the process of continuously steady removal of NO were investigated. The mechanism of this process was proposed and the effect of electron donor e.g. glucose and Fe(II)EDTA on the reduction of Fe(II)EDTA-NO was investigated. This study showed that reduction of NO in aqueous Fe(II)EDTA solutions was related to the concentration of glucose. The NO reduction rate was effected by the presence of Fe(II)EDTA, it was fast when the glucose was enough. Glucose played an important role during the reduction of Fe(II)EDTA, little Fe(II)EDTA was oxidized while glucose was existed. The concentration of cell increased in the condition of glucose as electron donor, while the Fe(II)EDTA acted as electron donor, it was down.
Keywords: electron donor; Fe(II)EDTA; Fe(II)EDTA -NO; microbial reduction