Abstract The purpose of this article is the study of natural medicine dichloromethane echinocystic acid ester modified and modified before and after the change insolubleExperimental methods for the use of methylene chloride to 1:7 ratio of echinocystic acid refine, Modified determine the optimum conditions, Determination of the product in ester compounds in the solubility, And application of infrared spectroscopy and electrospray mass spectrometry on the reaction product of the structural determination. Echinocystic acid modified determine the optimum conditions are as follows: The reaction temperature is at room temperature, the reaction time is eight hours, the reaction is a catalyst for sodium carbonate. Through the solubility experiment, we found that the solubility of modified significantly increased. By IR detection and mass spectrometry testing, experiments have proved the existence of product. Conclusion: dichloromethane can be effectively modified echinocystic acid, used infrared spectroscopy and mass spectrometry to detect changes in very good.
Keywords: Echinocystic acid Modified drug HPLC IR ESI- MS