响应面法优化葛根黄酮提取

化工论文编号:HG149  论文字数:12488,页数:30

 目  录
中文摘要 I
英文摘要 II
目录 III
1. 绪论 1
1.1  葛根介绍 1
1.2  葛根的主要有效组分 1
1.3  组分功效 2
1.4  葛根药理作用 3
1.5  黄酮性质及效用 5
1.6  葛根黄酮的提取方法 6
1.7  葛根黄酮的检测方法 7
1.8  葛根发展前景 7
2. 实验部分 8
2.1  实验原料、试剂与仪器 8
2.2  提取操作及分析过程 8
2.3  标准曲线绘制 9
3. 实验结果与讨论 11
3.1  单因素考察 11
3.2  响应面法优化葛根黄酮提取工艺Box-Behnken法 16
3.3  响应面法确立最佳工艺条件 16
4. 总结与展望 23
致谢 24
参考文献 25

摘 要：为优化葛根黄酮提取工艺，以葛根黄酮提取率为指标，对葛根黄酮提取过程中溶剂乙醇质量分数、提取时间、固液比、提取温度、pH、提取次数、重复性以及回收溶剂再利用等因素进行了考察。结果表明葛根黄酮的较优提取工艺为溶剂乙醇质量分数为70%，提取时间为120min，固液比为1:10，提取温度为70℃，pH=5，提取次数1次；浸提操作重复性好，溶剂回收再利用可行并且溶剂的回收率高达94.3%。在此基础上，通过Box-Behnken方法研究了固液比、提取温度、溶剂乙醇质量分数对葛根黄酮提取率的影响，建立数学模型，以确定提取葛根黄酮的最优条件。通过模型的响应曲面图及其等高线图，对影响葛根黄酮提取率的关键因素及其相互作用进行探讨，得到的优化工艺参数为：固液比为1:11，提取温度为78℃，溶剂乙醇质量分数为66%。在上述条件下提取效果最佳，葛根黄酮的提取率为2839.78μg/g。
 关键词：葛根黄酮；提取工艺；溶剂回收；响应面分析
 
Abstract: In order to optimize the extraction process of the Puerarin flavonoids,
Puerarin flavonoids yield was taken as an index, the quality score of solvent ethanol, extraction time,material-water ratio, extraction temperature,pH,extraction times, repeatability, recovery and reuse of solvent in the extraction process were studied. The results showed that the quality score of the ethanol was 70%, the extraction time was 120min, the material-water ratio was 1:10, the extraction temperature was 70℃, the pH was 5,the extraction times was 1; repeatability for extraction operation was good, recycling solvents was feasible and the solvent recovery was up to 94.3%. On this basis, the conditions of extracting Puerarin flavonoids were optimized using response surface mothod (RSM). Experimental factors and their levels were determined by one-factor tests.Subsequently, the Box-Benhnken experimental design with 3 factors and 3 levels was performed, and the factors influencing the technological were estimated by quadratic regression analysis with interaction terms. Response surface and contour were finally graphed with the extraction rate as the response value.The results showed that the optimal conditions of flavonoids extration were obtained as follows: the material-water ratio was 1:11, extraction temperature was 78℃，the quality score of solvent ethanol was 66 % and the extraction rate could be up to 2839.78μg/g.
Keywords: Puerarin Flavonoids; Extraction process; Solvent recovery;
 Response surface method