Uniform design and its application in studying and optimizing new drugs
Abstract: Uniform design has the characteristics of fewer experimental times, and the level of factors which can be appropriately adjusted, so it is a powerful tool for choosing optimal conditions, especially in looking for the best experimental conditions, the best chosen ratio and so on. Uniform Design had a wide range of applications in pharmaceutical research, mainly used in areas such as drug synthesis, drug analysis, the Pharmacy Group and the Optimization pharmacological experiments. The article introduces the method of experimental design and discusses the feature and application of uniform design on studying new drugs, as well as the designing of uniform design form.
The objective is to optimize the extracting technology of Si junzi Decoction. The optimal extracting technology of Si junzi Decoction was selected using Ginseng saponins and total polysaccharides as an index by a uniform design method. The results indicated that the optimal conditions were as follows：the concentration of alcohol was 10﹪(v／v)，the solvent quantity was 13 times as much as the drug quantity，the soak time was 14 hours and the reflux time was 2 hours. We can obtain the conclusion that the optimized procedure of uniform design succeeded in the optimal extracting technology of Si junzi Decoction. It is simple and easy to do, and suitable for practical production.
Key word: uniform design; orthogonal design; experimental scheme; studying drugs；optimum design
