Abstract: Using the silt from concentrate pool as an inoculum, phosphate reducer organisms which deoxidize phosphate into PH3 were cultured in a continuous stirred reactor. The functional bacterium was identified through traditional and modern isolation and identification methods. The taxonomic position was ascertained based on the investigation of configuration, physiological and biochemical properties, and 16S rDNA analysis. The activity in substrate degradation of the bacterium was investigated using the anaerobic reactor under anaerobic condition, which showed that NL is a heterotrophic dephosphorization bacteria under anaerobic condition. The 16S rDNA analysis indicated the strain had a 98.2% of homology with Pseudomonas aeruginosa. Its best carbon source is glucose and sodium acetate, and the best nitrogen source is ammonium chloride、nitrate and peptone. However, cellulose is unfit for carbon. The best initial pH value is 6.5~7.0, the optimum temperature is 30~35 ℃. The activity of microorganism decreased when the temperature was at 4 ℃ or above 35 ℃. The addition of organic phosphorus and calcium had distinct influence on the phosphorus removal, but the addition of reducer-sulfide had not apparent effect on the phosphorus removal.
Keywords: anaerobic dephosphorization; anaerobic dephosphorization bacteria; phosphate deoxidization; phosphine