疣粒野生稻来源的水稻抗白叶枯病单染色体片段渗透系的筛选

生物工程论文编号:SW195  论文字数:10576,页数:24

目  录
摘要 I
Abstract. II
目  录 III
1.绪论 1
1.1 水稻白叶枯病的致病及其抗病机理 1
1.2 水稻白叶枯病的病理基础知识及其对水稻的危害 2
1.2.1水稻白叶枯病的发病症状 2
1.3 水稻抗白叶枯病研究进展 3
1.4 分子标记的研究 4
1.4.1 SSR技术的特点 5
1.4.2 STS标记（sequence tagged site，SSR序标位) 6
1.4.3 SSR标记（simple sequence repeats,SSR 简单重复序列） 6
1.4.4 SSR 标记技术的基本原理 6
1.4.5 SSR及STS 标记技术程序 7
2.实验内容 7
2.1实验材料和方法 7
2.1.1 实验材料 8
2.1.2 水稻DNA的提取 9
2.1.3 制胶、电泳及检测 9
2.1.4 DNA片段的PCR扩增 10

3.结果与讨论 11
3.1 亲本电泳对照图 12
3.2 讨论 14
4.总结与展望 14
致谢 16
参考文献 17
附录 19

摘要：疣粒野生稻对水稻白叶枯病高抗，通过体细胞杂交将其抗性导入高产栽培稻大粒香中。经QTL定位，将高抗的杂交后代的抗性位点定位到1,3,5号三条染色体上，为了对这些抗性位点精细定位并克隆抗病基因，经多代回交构建了抗病杂交后代与感病亲本的高代回交群体，利用这些群体结合QTL连锁的分子标记进行包含抗性位点的单染色体片段渗透系的筛选，为抗性QTL位点的精细定位及基因克隆奠定基础。本实验就是对杂交群体中1号染色体上包含抗性QTL位点在内的单染色体片段渗透系的筛选。先通过电泳消除495个个体中3号及5号染色体上的抗性影响，使其拥有纯合的感病基因，继而对1号上的抗性位点进行研究，筛选包含抗性QTL位点在内的单染色体片段渗透系。
关键词：水稻；单染色体片段；白叶枯病

Abstract： wild rice with high resistance to rice bacterial blight, by somatic cell hybridization to brought its high resistance into cultivated rice in a large grain incense. By QTL location, the high resistance of the hybrid offspring of the resistance loci in No. 1,3,5. in order to fine mapping of these resistance loci and cloning of resistance genes,was constructed by resistance disease and susceptible hybrids groups, these groups combined use of molecular markers linked to QTL for resistance loci contain a single chromosome segment penetration system of selection for the resistance locus QTL Fine Mapping and Cloning basis. The experiment is on chromosome 1 hybrid population's resistance gene screening. First by electrophoresis elimination of 500 individuals NO.3 and NO.5 Effects of resistance on chromosome so that it has a homozygous susceptible genes. Then on the NO.1's Resistance to research.
Key words: Rice; single chromosome segment; blight