Abstract A crystallization method is applied to separate asiaticoside from total glucosides of Centella asiatica. Through the study of different solubility of total glucosides of Centella asiatica under a variety of melting-out agent and the resolution in different solvents - melting-out agent system were observed. More than 98.1% purity asiaticoside was obtained. The method is simple process, lower the prime cost and energy consumption, reducing pollution emissions.