Abstract: Industrial gardenia yellow from the traditional method of extracting ethanol is used, this method of extracting long cycle and low efficiency; Ultrasonic extraction have high yields and save solvent, time-saving advantages. This paper mainly dealt with the extraction conditions of gardenia yellow, Gardenia fruit as raw material to extract isolated from the gardenia yellow-and Gardenia Effect products. From the temperature, liquid than expected, the extraction time, and other conditions, the conditions do all single-and multi-factor factor comprehensive experiment to find out gardenia yellow pigment extracted the best conditions. Determined through experiments ethanol extraction and ultrasonic cycle from gardenia yellow pigment prepared by the technical conditions. Through experiments, the optimum extraction conditions of ethanol extraction and ultrasonic extraction were obtained. The optimum conditions of ethanol extraction were ethanol/water (5:5),gardenia fruit :solvent =1:10. extraction time 3 days, Under these conditions pigment have a higher extraction rate. The optimum conditions of ultrasonic extraction were ethanol/water (5:5), extraction time 30 min, extraction power 500W, gardenia fruit :solvent =1:5. Under these conditions pigment have a higher extraction rate. we detected Gardenia active ingredients in liquid chromatography method.