Abstract: In order to detect the effects of sterilization equipment sterilization,using the spores of bacillus shrinking as biological indicators. Most of home and abroad with soild and liquid culture, but in this study ,semi-soild medium filter method to develop atrophy and spore of Bacillus.This method not only to maintain soild and liquid culture has the advantage of overcoming their lack of, while increasing the formation rate of shrinkage of Bacillus spores and the number of total bacteria and spores. Of nutritional condition on the spore yield. The results showed that:the optimal sporulation medium composition for(g/L):10g glucose、10g yeast extract、5g NaCl、0.6g MnSO4·7H2O、pH 7.0.Cultured under these conditions 30℃,after 24h incubation,the number of spores can reach 6.03×108mcfu/ml,sporulation rate reached82.59%.
Keywords: Bacillus atrophaeus Semi-soild culture Membrane culture Sporulation rate