茶树菇深层发酵条件的优化

生物工程论文编号:SW102  论文字数:14197,页数:27

目  录
中文摘要 Ⅰ
英文摘要 Ⅱ
目录 Ⅲ
1. 绪论 1
 1.1 食用菌深层发酵研究概况 1
 1.2 真菌多糖的研究概况 3
 1.3 茶树菇概述 7
2．实验部分 9
 2.1 实验材料和设备 9
 2.2 实验方法 10
3 实验结果与讨论 12
 3.1 碳源对茶树菇菌丝体干重及胞外粗多糖产量的影响 12
 3.2 氮源对茶树菇菌丝体干重及胞外粗多糖产量的影响 13
 3.3 两种无机盐浓度对茶树菇菌丝体干重及胞外粗多糖产量的影响 13
 3.4 正交试验优化培养基组成 14
 3.5 摇瓶装液量对茶树菇菌丝体干重及胞外多糖产量的影响 16
 3.6 小结 17
4 总结与展望 17
致　　谢 19
参考文献 20
（附图片）   

 摘 要:本研究主要对茶树菇深层发酵培养基和发酵条件的优化,胞外多糖的提取以及其最优发酵条件进行了研究。
  采用摇瓶发酵法研究不同碳源、氮源、无机盐浓度对茶树菇菌丝体干重及胞外多糖产量的影响；并用正交试验获得了茶树菇深层发酵的最优培养条件。最优的培养基组成为玉米粉2%、酵母粉0.3%、KH2PO40.2%、MgSO40.05%。同时看到当碳源、氮源浓度再增高时,菌丝体干重和胞外多糖产量都呈下降趋势,表明高浓度的碳源、氮源对茶树菇的深层发酵有抑制作用。另外,茶树菇胞外多糖产量和菌丝生长成一定的正比关系。
 同时还进行了300mL三角瓶的装液量试验,得到当装液量为80mL时,有最大菌丝体干重和胞外多糖产量。
 关键词:茶树菇；正交试验；深层发酵；条件优化；装液量

 Agrocybe cylindracea fermentation conditions of optimization
 Abstract:This research mainly for Agrocybe cylindracea fermentation medium and fermentation conditions optimization, exopolysaccharide extraction and the optimal fermentation conditions.
 This study researchs different carbon sources, nitrogen sources, inorganic salt concentration influences the dry weight of mycelium Agrocybe and Exopolysaccharide Yield by using shake flask fermentation. and orthogonal test was exerted to study Agrocybe cylindracea’s fermentation of optimal condition.And fermentation of optimal condition is: corn meal 2%,dusty yeast 0.3%, KH2PO4 0.2%, MgSO4 0.05%.While when the carbon source, nitrogen concentration increased further, the dry weight of mycelia and exopolysaccharide production both decrease, indicating that high concentrations of carbon and nitrogen sources on the Agrocybe inhibit fermentation. In addition,we can see that Agrocybe Exopolysaccharide production and mycelial growth into a positive relationship.
 At the same time,we have a test of liquid medium volume about 300mL shake flask。We find that the volume of liquid 80mL it have maximum dry weight of mycelium and production of exopolysaccharide.
Keywords：Agrocybe; orthogonal test; submerged fermentation; Optimization; loaded liquid