培养基成分对枯草芽孢杆菌黑色变种芽孢形成率的影响

生物工程论文编号:SW101  论文字数:10637,页数:24

目  录
中文摘要： I
外文摘要： II
目  录 III
1. 绪论 1
1.1枯草芽孢杆菌简介 1
1.2枯草芽孢杆菌的应用 1
1.3 研究目的 5
2．实验部分 6
2.1实验材料 6
2.2实验方法 7
3结果与讨论 10
3.1生长曲线 10
3.2不同碳源对芽孢产量的影响 11
3.3不同氮源对芽孢产量的影响 12
3.4无机离子对芽孢产量的影响 12
3.5不同Mn2+ 浓度对芽孢产量的影响 13
3.6正交实验结果 14
3.7小结 15
4总结与展望 17
致  谢 19
参考文献 20

摘 要：本论文主要为了提高枯草芽孢杆菌黑色变种ATCC9372的芽孢形成率，以利于枯草芽孢杆菌制剂在生活生产中的应用。为此，研究了枯草芽孢杆菌的生长曲线，碳源，氮源，无机盐及生长因子MnSO4·H2O对其总菌数和芽孢形成率的影响。生长曲线是通过比浊法在波长为530nm下测试所得。枯草芽孢杆菌总数是通过倾注法培养，平板计数所得。枯草芽孢杆菌的芽孢数是在菌样经过80℃水浴20min后，通过倾注法培养，平板计数所得。首先通过单因子实验，选择芽孢形成率比较高的，碳源（葡萄糖和乳糖），氮源（蛋白胨和小牛浸膏）和无机盐NaCl及浓度最适的Mn2+，最后通过正交实验。最终所得结果表明枯草芽孢杆菌ATCC9372在14h时达到最大生长点，其最适合的培养基成分为蛋白胨5g/L，乳糖5g/L，NaCl5g/L，Mn2+浓度为2.4mmol/L。

关键词：枯草芽孢杆菌; 芽孢形成率; 培养基; 生长曲线

 Medium Component Optimization for Spore Production of Bacillus subtilis var. niger ATCC 9372

Student’s name: Bijing bijing BIJINGApan?    Pan    Advisor: Wei Peilian
（School of Biological and Chemical Engineering Zhejiang University of Science and Technology）

Abstract: This article in order to improve the strain Bacillus subtilis var. niger ATCC 9372, spores formation rate bacillus subtilis agents in the application of life. Therefore, study the Bacillus subtilis growth curve, the source of carbon, nitrogen source, inorganic salt and growth factors of its total MnSO4·H2O count and spores formation rate. Through the growth curve is 530nm for under test in the wavelength. Bacillus subtilis is through the method of total into training, plate counting income. Bacillus subtilis is the spores in bacteria in water sample after 20min at 80℃, through cultivating, plate counting into law. First to test the single factor experiment, choose through spores are formed rate is high, carbon source (glucose and galactose) and nitrogen source (Peptone and beef extract) and NaCl and the optimal concentration of Mn2+, finally through orthogonal experiment. Final results show Bacillus subtilis ATCC937214h in its maximum growth, while the most suitable for medium composition Peptone 5g/L, 5g/L, lactose NaCl5g/L, and the concentration of Mn2+ is 2.4 mmol /L.
Key words：Bacillus subtilis, Spores production, Medium, The growth curve