利用廉价原料发酵法生产虾青素

生物工程论文编号:SW057  论文字数:10895,页数:25

目  录
中文摘要 I
英文摘要 II
目录 III
1. 绪论 1
 1.1  虾青素的结构 1
 1.2  虾青素的性质 2
 1.3  虾青素的来源 2
 1.4  虾青素的功能 3
 1.5 虾青素的应用 4
 1.6 天然虾青素的安全性 5
2. 实验部分 6
 2.1 酵母培养工艺 6
 2.2  实验材料 6
 2.3  实验方法 7
3. 结果与讨论 8
 3.1  种龄和接种量的选择 8
 3.2 培养基最优碳源的选择 9
 3.3 培养基最氮碳源的选择 11
 3.4  正交设计试验 13
 3.5  酵母生长曲线的测定 14
4．总结与展望 16
 4.1  总结 16
 4.2  展望 16
致谢 18
参考文献 19

摘 要：虾青素是一种酮式类胡萝卜素，可作为抗氧化剂、色素和食品添加剂等,在医药、食品、水产养殖等方面具有非常广泛的应用。本文概述了虾青素的结构、性质、来源、功能、应用、发展前景等。本次实验用红法夫酵母发酵生产虾青素，它是天然可产虾青素的主要微生物之一，研究通过红法夫酵母发酵法生产得到虾青素的方法以及生产条件是本次实验的主要目的。研究方法主要是先通过单因素试验发现培养基的最佳氮源是尿素，最佳碳源是蔗糖，最佳的接种量是6%。在此基础上用正交试验设计法对发酵培养基的成分进行了初步优化，为进一步工业化生产虾青素提供参考依据。

关键词：红法夫酵母；虾青素；廉价原料；发酵

Use of cheap raw materials produced by fermentation of astaxanthin

Abstract: Astaxanthin is a carotenoid keto form can be used as antioxidants, pigments and food additives, medicine, food, aquaculture has a very wide range of applications. This article outlines the structure of astaxanthin, nature, origin, function, application, development prospects. The experimental production of red yeast Phaffia astaxanthin, it is natural astaxanthin can be produced one of the main microorganisms studied by yeast fermentation of Phaffia astaxanthin production by the method and the production conditions of this experiment The main purposes. Research method is to first find the single factor test medium, the best nitrogen source is urea, the best carbon source is sucrose, the best inoculum is 6%. On this basis, orthogonal experimental design method on the fermentation medium components were initially optimized for the industrial production of astaxanthin to further provide a reference. Keywords: Phaffia rhodozyma; astaxanthin; cheap raw materials; fermentation