Abstract: Astaxanthin is a high-value-additive,which has wide applications in food,medicine and cosmetics.Phaffia rhodozyma is one of the microorganisms which produce natural astaxanthin.The methoud of astaxanthin fermentation by Phaffia rhodozyma and the conditions of production was introducted and studied in paper. single factor design experiments shows that the best nitrogen is (NH4)2SO4,the best carbon is glucose. Two orthogonal experiments showed that the optimalmediun was composed of1.5 g/L(NH4)2SO4, 30 g/L glucose and 1.19g/L yeast extract. Experiments showed that in the 0-22 hours, yeast cell concentration did not change ,22-49 hours, the cells have grown geometrically, 53 hours later, the cell concentration has been relatively stable. PH decreased gradually. In this paper , the research advances and methods in breeding and fermentation optimization of Phaffia rhodozyma are summarized.Furthermore , strategy was proposed to solve the problems in large2scale commercial production of astaxanthin.
Key words : Phaffia rhodozyma ; astaxanthin ; fermentation