Abstract:Through the research about the fermentative conditions of β-carotene by Blakeslea trispora,with a view to further disquisition about the circumstances of the optimal β-carotene fermentation medium. This thesis mainly from the content of the fermentation medium and the fermentation conditions.First,study the infection about the component of the medium, and know the medium composition on Blakeslea trispora effect of β-carotene. Initially indentified as a carbon source to corn starch, soybean power as a source of fermentation medium. Secondly, through change the constant variables factor to determine the final composition of the ratio of the various media, such as nitrogen concentration of 3%,carbon concentration 3%,medium pH value of 7.0,vegetable oil to 35ml/L, , KH2PO4 2g / L, MgSO4 1g / L, vitamin B1 0.03g / L situation.