Research on Cultivating of the Camptotheca Acuminata Hairy Roots and the Secondary Metabolite Camptothecme in the Culture Medium
Abstract: The camptotheca acuminata hairy root raise not can only produce the camptotheca acuminata alkali, moreover may secretion and so on two metabolite like camptotheca acuminata alkali to the culture medium, the use modern biological technology and the modern isolation technique comprehensive development camptotheca acuminata and the camptotheca acuminata alkali, this is the camptotheca acuminata resources protection development new breakthrough. The present paper carries on the raise first to the camptotheca acuminata roots of the hair, then in the use chloroform extraction camptotheca acuminata culture medium the camptotheca acuminata alkali and so on effective component, and examines the law with HPLC to examine its effective component, the examination discovers the camptotheca acuminata secretion in the culture medium secondary metabolite besides the camptotheca acuminata alkali, the hydroxyl camptotheca acuminata alkali, the white birch fatty acid, but also includes other effective components. Other chloroform extraction process and the resin adsorption law to added the sign fluid blank culture medium to carry on the extraction the opposite experiment, had proven the resin concentrated in the culture medium the micro camptotheca acuminata alkali effect to be better than the chloroform extraction effect, moreover concentrated the type must compared to chloroform extraction many. This experiment will take the adsorption material for later with the resin, concentrated the camptotheca acuminata alkali from the camptotheca acuminata roots of the hair system's culture medium to make the very good upholstery.
Key word: camptotheca acuminata alkali Camptotheca acuminata roots of the hair culture medium Chloroform extraction HPLC examination