木霉菌对节瓜枯萎病的生物防治作用

食品科学论文编号:SP021 论文字数:7870,页数:17

 目    录
1  前言 1
2  材料与方法 2
2．1  木霉菌的分离纯化 2
2．1．1 木霉菌的分离 2
2．1．2 木霉菌的纯化和保存 2
2．2  拮抗镰刀菌的木霉菌筛选 2
2．2．1 供试菌株和材料 2
2．2．2 对峙培养 2
2．3  木霉菌生物学测定 3
2．3．1 菌种来源 3
2．3．2 环境因子对分生孢子萌发的影响 3
2．3．3 环境因子对菌丝生长和产孢的影响 3
3  结果及分析 4
3．1  木霉菌的分离纯化 4
3．2  拮抗镰刀菌的木霉菌筛选 4
3．3  木霉菌生物学测定 6
3．3．1 环境因子对孢子萌发的影响 6
3．3．2 环境因子对菌丝生长和产孢量的影响 7
4  结论与讨论 10
4．1  木霉菌的分离纯化 10
4．2  拮抗镰刀菌的木霉菌筛选 10
4．3  木霉菌生物学测定 10
4．3．1  关于环境因子对孢子萌发的影响 10
4．3．2  关于环境因子对木霉菌生长速度和产孢量的影响 11
参 考 文 献 12
英 文 摘 要 13
致       谢 14

摘    要
    将从土壤中分离出来的8株木霉菌（Trichodema spp.），与节瓜枯萎病原菌进行对峙培养，筛选出拮抗节瓜枯萎病菌镰刀菌的优良木霉菌T04-004菌株，该菌株对节瓜枯萎病菌的抑制率达90%以上。对生防菌木霉菌T04-004进行生物学特性测定，结果表明，在Richard培养液中，T04-004菌株孢子萌发需要一定的营养，其中以PD培养液最好，并且在PDA培养基上菌丝生长最好，产孢量最大。T04-004菌株孢子萌发，菌丝生长和产孢的温度范围为15℃～40℃；T04-004菌株孢子萌发，菌丝生长和产孢的pH值范围为2～10；光照对菌丝的生长影响不显著，但对产孢量的影响较大。
关键词：木霉菌 节瓜枯萎病原菌 拮抗作用 生物学特性

 Biological control Effect of Trichodema on Fusarium oxysporum f.sp.benincasae
                     
Abstract：    8 Trichodema were collated from the soil, carries on confronts the Fusarium oxysporum f.sp.benineasae, screens the fine Trichodema T04-004 that oppresses Fusarium oxysporum f.sp.benineasae, This strain to the Fusarium oxysporum f.sp.benineasae 's suppression rate achieved above 90%. The biological characterizations of Trichoderma viride T04-004 were screened and identified. The results illustrated that the spore germination of T04-004 strain needs certain nutrition, and the PD nutrient fluid is the best. The strain T04-004 grows well in the PDA culture medium, including the hypha and spore production. The temperature range of spore sprouts, hypha growth and spore production is 15℃~40℃, and the range of pH-value is 2~10. The illumination is not remarkable influence to hypha growth, but significantly affect on the spore production.
Key words: Trichodema; Fusarium oxysporum f.sp.benincasae; antagonism; biological characteristic