Abstract
Chondroitin sulfate is a mucopolysaccharide acid, usually in the form of a glycoprotein found in animals. Chondroitin sulfate is aminocaproic acid and glucose sugar as the basic unit consisting of polymers, molecular weight of 1 × 104 ~ 5 × 104D.
Papers to bovine tracheal cartilage as raw materials, use of buffer solution - enzyme - alcohol-law and chondroitin sulfate in the rate of key indicators on the type and solvent extraction conditions and other factors on the effects of chondroitin sulfate from the impact. The results showed that Na2HPO4 used as a buffer solution extracts, to feed than 1:12 (w / v), extraction temperature of 40 ℃, extraction time for 36 h, alcohol-alcohol concentration of the system 70% (v / v), standing at room temperature 1 h after the centrifugal (500 r • min-1) 5min, the above methods and conditions for the extract of chondroitin sulfate, chondroitin sulfate content of up to 61% (w / w) , Chondroitin sulfate in the rate was 21.5% (w / w).
Papers on ethanol precipitation purification of the effect of chondroitin sulfate. The results showed that when extracts chondroitin sulfate solution solids concentration of 18% (w / v), NaCl concentration of 3% (w / v), pH 6.4, the system of ethanol-alcohol concentration of 70% (v / v), precipitation repeated four times, chondroitin sulfate content of up to 93.23% (w / w), compared with the original extracts (chondroitin sulfate content of 61%, w / w) of the increase in chondroitin sulfate A 52.83 percent, chondroitin sulfate in the rate of 45.00% (w / w).