The use of biological indicator of ozone disinfection
Abstract
Ozone disinfection and sterilization technology for its effective, easy to use, for operators and workplaces no danger, now widely used for the pharmaceutical industry, mainly for the one hundred thousand grade on the environment under the conditions of ozone disinfection, sterilization. In the microbial challenge experiments, the traditional test of ozone disinfection is used to detect Bacillus subtilis, but the results show detection of Bacillus subtilis as an indicator of the existence of more defects, the bactericidal rate was less than a microbial challenge experiments standards. To improve detection sensitivity, were investigated in this experiment in order to Staphylococcus aureus and Bacillus subtilis as an indicator micro-organisms, at different time of the ozone disinfection, ozone concentration, temperature and humidity conditions, the environment to detect the amount of biomass residues bactericidal effect. Experimental data show that the disinfection of Staphylococcus aureus in time is 2 hours, the ozone concentration in the 10-15ppm between the temperature of 18-26 ℃, humidity between 60 ~ 65%, its detection sensitivity better than Bacillus subtilis, it Staphylococcus aureus can be selected after ozone disinfection of environmental testing.
Key words: aureus subtilis; ozone; microbial challenge test