Preliminary study of hydrolyzed protein of owls
with immobilized enzyme
Abstract: Immobilized enzyme technology is extracted from the body's enzymes, using artificial methods fixed on the carrier. Compared with the free enzyme, immobilized enzyme can be used to duplicate greatly reduce the production cost. This paper mainly discusses some problems of using immobilized neutral proteinase hydrolysis owls process.Research of the effects of hydrolysis temperature, solution pH, reaction time, enzyme concentrate on immobilized neutral proteinase hydrolysis owls. By orthogonal experimental optimum hydrolysis conditions is: hydrolysis temperature 50℃, the solution pH7, hydrolysis time 5h, enzyme concentration 1.5%. Comparison of the vitality of the free enzyme and the immobilized enzyme when they hydrolyse owls. Also compare the vitality of neutral relatively immobilized enzyme and immobilized acid enzyme when they hydrolyse owls.
Key words: Immobilized enzyme; Hydrolysis; Owls protein; Orthogonal