Study on Establishing of Clone and In vitro Flowering Induction of Bougainvillea spectabilis Willd.
Abstract: The sterilized indefinite shoots induced from the axillary’s buds of Bougainvillea spectabilis Willd. stem were used as the material, the effects of varies treatment on establish of germfree system and flower inducing such as the mode of inoculation of explants, external hormone, nutrition environmental condition and so on were studied respectively. These results were indicated that, slit an incision vaccinate in MS+6-BA0.05mg/L, propagate is the highest, about 4.06. In MS+6-BA0.05mg/L, under natural lighting and temperature, value of pH about 5.6, the concentration of sucrose and the agar is 3.0% and 0.6% separately, dispersion of in vitro flowering is 45.83%.
Key words: Bougainvillea spectabilis Willd.; clone; in vitro flowering